Proline-Proline-Glutamic Acid (PPE) Protein Rv1168c of Mycobacterium tuberculosis Augments Transcription from HIV-1 Long Terminal Repeat Promoter
نویسندگان
چکیده
منابع مشابه
The interconversion of glutamic acid and proline.
Oxidation of proline to glutamic acid is presumed to proceed via the intermediate Al-pyrroline-5-carboxylic acid (l-3) and therefore should require the participation of an enzyme system oxidizing the latter compound. We have previously reported the existence of such an enzyme in rat liver mitochondria (4). This communication describes the partial purification and some properties of a pyridine n...
متن کاملThe N-terminal domain of Mycobacterium tuberculosis PPE17 (Rv1168c) protein plays a dominant role in inducing antibody responses in active TB patients
The PPE (proline-proline-glutamic acid) proteins of Mycobacterium tuberculosis are characterized by a conserved N-terminal domain of approximately 180 amino acids and variable C-terminal domain. Since last decade, these proteins have gained much importance in the serodiagnosis of tuberculosis (TB) as they act as a source of antigenic variation. We have demonstrated earlier that one of the PPE p...
متن کاملAssociation of strong immune responses to PPE protein Rv1168c with active tuberculosis.
Accurate diagnosis of tuberculosis (TB) infection is critical for the treatment, prevention, and control of TB. Conventional diagnostic tests based on purified protein derivative (PPD) do not achieve the required diagnostic sensitivity. Therefore, in this study, we have evaluated the immunogenic properties of Rv1168c, a member of the PPE family, in comparison with PPD, which is routinely used i...
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23 Protein engineering is commonly used to improve the robustness of enzymes for 24 activity and stability at high temperatures. In this study, we identified four residues 25 expected to affect the thermostability of Streptomyces sp. S9 xylanase XynAS9 26 through multiple sequence analysis (MSA) and molecular dynamic simulations 27 (MDS). Site-directed mutagenesis was employed to construct five...
متن کاملThe PPE18 of Mycobacterium tuberculosis interacts with TLR2 and activates IL-10 induction in macrophage.
The pathophysiological functions of proline-glutamic acid (PE)/proline-proline-glutamic acid (PPE) family of proteins of Mycobacterium tuberculosis are not well understood. In this study, we demonstrate that one of the PPE proteins, PPE18 can stimulate macrophages to secrete IL-10, known to favor a Th2 type response. The recombinant PPE18 was found to specifically interact with the TLR2 leading...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 2012
ISSN: 0021-9258
DOI: 10.1074/jbc.m111.327825